固定化脂肪酶TL IM
Lipase
CAS: 9001-62-1
Molecular Formula: C11H9N3NaO2+
固定化脂肪酶TL IM - Names and Identifiers
固定化脂肪酶TL IM - Physico-chemical Properties
| Molecular Formula | C11H9N3NaO2+ |
| Molar Mass | 238.19783 |
| Density | 1.2 |
| Water Solubility | It is soluble in water. |
| Solubility | H2O: 2mg/mL, hazy with insoluble particles, faintly yellow |
| Vapor Presure | 0.004Pa at 25℃ |
| Appearance | Near white freeze-dried powder or creamy yellow powder |
| Color | yellow-brown |
| Merck | 13,5533 |
| Storage Condition | 2-8°C |
| Stability | Moisture sensitive. Incompatible with strong oxidizing agents. |
| Sensitive | Humidity sensitivity |
| MDL | MFCD00131509 |
| Physical and Chemical Properties | Nearly white freeze-dried powder or milky yellow powder, soluble in water, the optimum temperature is 35 ~ 370C. |
| Use | Vensolide (carbavir, abacavir and peramivir intermediates) |
固定化脂肪酶TL IM - Risk and Safety
| Risk Codes | 20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36/37 - Wear suitable protective clothing and gloves. S36 - Wear suitable protective clothing. |
| WGK Germany | 3 |
| RTECS | TO9776500 |
| FLUKA BRAND F CODES | 3-10-21 |
| TSCA | Yes |
| HS Code | 35079020 |
固定化脂肪酶TL IM - Nature
Open Data Verified Data
This product is white or milky yellow lyophilized powder, soluble in water. Those made from Aspergillus oryzae may be in the form of powder or fat. The main component is an enzyme that breaks down fats. Its basic function is to hydrolyze triglycerides to glycerol and fatty acids. It can only function in a heterogeneous system, I .e. at the interface of oil and water, and has no effect on uniform dispersion or water-soluble substrates, even if it has an effect, it is extremely slow.
Last Update：2024-01-02 23:10:35
固定化脂肪酶TL IM - Preparation Method
Open Data Verified Data
prepared from porcine pancreas. 3kg of pig pancreas was ground and then subjected to secondary deesterification with chloroform and butanol, followed by ethanol extraction and freeze-drying to obtain 365g of pancreatic lipase.
Last Update：2022-01-01 11:10:47
固定化脂肪酶TL IM - Introduction
& & molecular weight: 20000~60000. & & Vitality: ≥ 30000u/g. & & loss on drying: ≤ 8.0%. & & properties: white-like or yellowish powder. It belongs to fat plus water decomposing enzymes. & & Optimum temperature: 35~37 ℃. & & absorbance coefficient (E1%280):13.3. & & Activator: Calcium (CA2 +).
Last Update：2022-10-16 17:29:45
固定化脂肪酶TL IM - Use
Open Data Verified Data
can be used as an enzyme preparation. It is mainly used in cheese manufacturing (degreasing and producing special flavor, with the highest dosage of lOOmg/kg), lipid modification, lipid hydrolysis, to prevent the deterioration of fats in some dairy products and chocolate. It is an excellent preparation for producing special flavor of milk chocolate and cream cake. The protein is added to break down the possibly mixed fat, thereby improving its foaming ability. The addition of lipase during wine brewing can promote the fermentation of wine and increase the aroma of wine.
Last Update：2022-01-01 11:10:47
固定化脂肪酶TL IM - Safety
Open Data Verified Data
lg, 5G packaging, sealed and dried at 4 °c.
Last Update：2022-01-01 11:16:47
固定化脂肪酶TL IM - Reference Information
| LogP | -1.3 at 20℃ |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Overview | Lipase (glyceryl ester hydrolase) belongs to the class of carboxyl ester hydrolases, triglycerides can be hydrolyzed stepwise to glycerol and fatty acids. Lipases are found in tissues of animals, plants, and microorganisms (e. G., molds, bacteria, etc.) that contain fat. These include phosphatase, sterol and carboxylesterase. Fatty acids are widely used in food, medicine, leather, daily chemical industry and other aspects. Lipases are widely found in animals, plants and microorganisms. lipase is a kind of enzyme with a variety of catalytic ability, which can catalyze the hydrolysis, alcoholysis, esterification, transesterification and reverse synthesis of esters, in addition to the activity of some other enzymes, such as phospholipase, lysophospholipase, cholesterol esterase, acylpeptidyl hydrolase activity (Hara; Schmid). The different activities of lipase depend on the characteristics of the reaction system, such as promoting ester hydrolysis at the oil-water interface, and enzymatic synthesis and transesterification in organic phase. |
| Source | the seeds of oil crops, such as castor bean, rapeseed, when oil seeds germinate, lipase can cooperate with other enzymes to catalyze the decomposition of oil and fat substances to produce sugars, and provide the necessary nutrients and energy for seed rooting and germination; the animal body contains more lipase in the pancreas and adipose tissue of higher animals, and contains a small amount of lipase in intestinal fluid, which is used to supplement the deficiency of pancreatic lipase on fat digestion, there is a small amount of butyoglyceroesterase in the gastric juice of predators.|
| properties | lipase, all known as triacylglycerol hydrolase, can catalyze the hydrolysis of ester bonds at the lipid-water interface, it can catalyze the hydrolysis, alcoholysis, esterification, transesterification and reverse synthesis of triacylglycerides and other water-insoluble esters. Lipase widely exists in animals, plants and microorganisms. Due to the variety of microorganisms, wide distribution, strong adaptability and fast evolution, lipase in microorganisms often has a wider temperature than lipase from animals and plants, advantages such as pH and substrate specificity. Microorganisms are the main source of industrial lipases. lipases are a class of hydrolases capable of acting on carboxylate bonds. The biological properties of lipases are the hydrolysis of triglycerides into diglycerides, monoglycerides, fatty acids and glycerol. In addition, in addition to the natural role of hydrolysis of carboxylic acid ester bonds, lipase can also catalyze the esterification, transesterification and transesterification in non-aqueous systems. These characteristics of lipase make it widely used in food, medicine, detergent, leather products, paper industry and biodiesel production. |
| enzyme preparation | lipase is one of the important industrial enzyme preparations, which can catalyze lipolysis, Ester exchange, ester synthesis and other reactions, widely used in oil processing, food, medicine, daily chemical industry. Different sources of lipase have different catalytic characteristics and catalytic activity. The large-scale production of lipase with transesterification or esterification function for organic phase synthesis is of great significance for enzymatic synthesis of fine chemicals and chiral compounds. lipase is a special ester bond hydrolase, which can hydrolyze glycerides (oils, lipids) and release fatty acids, diglycerides, monoglycerides and glycerol at different stages. The fatty acids produced by hydrolysis can be titrated with a standard alkaline solution and the enzyme activity expressed as a titration value. lipase is widely distributed in microorganisms, and the main producing bacteria are mold and bacteria. There are 33 different sources of lipases that have been published for triglyceride processing, of which 18 are from molds and 7 from bacteria. |
| Application | lipase is widely used in industrial production. For example, in the food industry, lipases are used for meat degreasing, flour improvement, oil modification, cheese processing, wine making and sauce processing. In the feed industry, the compound enzyme of lipase and other carbohydrate degrading enzymes can effectively improve the utilization rate of feed. Lipases are also widely used in the biological resolution of chiral compounds in the pharmaceutical and fine chemical industries. In the paper industry, lipase can be used for enzymatic removal of resin from wood pulp. In the biodiesel industry, lipases can be used to catalyze the transesterification of oils and fats with transesterified alcohols for the enzymatic synthesis of biodiesel. Lipases are also widely used in the washing industry, leather processing and textile industries. |
| preparation | a method for extracting and purifying lipase by immobilized horse antibody, comprising the steps of: S1: A single colony of lipase-producing Pseudomonas ATCC21808 was placed in a 250ml shake flask containing 25ml of Shake flask culture solution and cultured for 20h at 28 °c and 200rpm, then, the fermentation broth was transferred to the fermentation broth according to the inoculation amount of 10%, and cultured at 28 ℃ and 200rpm for 68H to obtain the fermentation broth. Each liter of Shake flask culture broth included: 5g of yeast extract, 5g of peptone, 10g of vegetable oil, the balance is water; PH value is 7.0; Each liter of fermentation broth includes: 10g of yeast extract, 5g of peptone, 0.5g of NaCl, 10g of vegetable oil, the balance is water; PH value is 7.0;S2: the fermentation broth was centrifuged, and the supernatant was collected to obtain a crude lipase enzyme solution. The centrifugation was carried out by using a high-speed refrigerated centrifuge at 4 ° C. And 10800G for 15min: immobilized horse antibody column on crude lipase enzyme solution, During the column loading, the lipase activity of the effluent was detected until the lipase activity flowed out, and the liquid loading was stopped; Then the column was washed with 0.1M disodium hydrogen phosphate-potassium dihydrogen phosphate buffer at pH 7.2, then eluted with 0.1m disodium hydrogen phosphate-citric acid buffer of PH 3 to collect the eluate to obtain purified lipase, the column is immediately washed to Neutral with 1m disodium hydrogen phosphate-potassium dihydrogen phosphate buffer of pH 8, and the eluate is also adjusted to pH 7 with the solution. The preparation method of the immobilized horse antibody column comprises the following steps: select 4 Mongolian horses (weight and corresponding age respectively: weight of 240 and age of 3 years, weight of 290 and age of 5 years, the weight is 320 and the age is 7 years old, the weight is 360 and the age is 10 years old), once every two weeks, a total of 12 weeks of immunization, each time 2mL, the dosage was 15mg lipase plus the same amount of Freund's complete adjuvant, and the multiple subcutaneous immunization was carried out. The antiserum was collected after the antibody titer was greater than 1:10000 by ELISA, after the supernatant was collected, the polyclonal antibody was purified by proteinA or proteinG affinity chromatography column; The polyclonal antibody was linked with the activated Sepharose 2B to obtain an immobilized horse antibody column; S4: polyethylene glycol was added to the purified lipase, The lipase was precipitated; Then centrifuged to collect the precipitate; The precipitate was lyophilized and dried to obtain lipase, and the enzyme activity was determined to be 15-250,000 units/G; Wherein, the centrifugation was performed at 4 ℃, 000g under the condition of centrifugation 8min. |
| toxicity | FAO/WHO regulations in, ADI is not restricted by animal tissue extraction, dosage is limited to GMP; ADI produced by Aspergillus oryzae is not specified. GRAS(FDA,§184.1027,2000). |
| Use limit | GB 2760 2002 (refers to Aspergillus oryzae carrying a lipase gene from Fusarium oxysporum): oil degumming 400I. ENU/kg triglyceride; Hydrolysis of lecithin, 10000LENU/k crude lecithin; Emulsification of egg yolk, 8000LENU/kg egg yolk; Others are limited to GMP. |
| Use | is commonly used in diagnostic enzymes. Quantitative analysis of serum triglycerides, prostaglandin esters, fat analysis, biochemical reagents. enzyme preparation. Mainly used for lipid modification, lipid hydrolysis and cheese manufacturing, can prevent chocolate and dairy products oil rancidity. Lecithin hydrolysis of the maximum use of 10000LENU/kg crude lecithin, other production needs according to the appropriate use. for biochemical studies, analysis of prostaglandin esters, triglycerides in serum, fat analysis. enzyme preparation. Mainly used in cheese manufacturing (degreasing and make the product produce special flavor, the maximum amount of 100mg/kg), lipid modification, lipid hydrolysis, in order to prevent some dairy products and chocolate in the fat failure. It is an excellent preparation for producing special flavor of milk chocolate and cream cake. Proteins are added to break down fats that may be incorporated therein, thereby increasing their foaming ability. commonly used in diagnostic enzymes; Determination of triglyceride in serum; Analysis of prostaglandin lipid; Analysis of fat Food Industry: good emulsification performance. The stability and fermentation resistance of dough are enhanced, the cooking quality and strength are improved, and the product volume is increased. With whitening effect, improve the quality of the skin of steamed bread products, internal color. It can improve the internal structure of steamed bread, improve the appearance and texture, and improve the taste. The shelf life of the product is prolonged, and the effect of re-steaming is good. The recommended dosage is 1.0-3.0g/100 of flour (10-30ppm). B) food flavoring: Through the decomposition of fatty acids in the raw materials, it is converted into substances with special aroma, which increases the flavor of the product. Such as liquor brewing, dairy flavor. c) biological fermentation and animal extract process for hydrolysis or degradation of oils and fats. 2.5d) in fillets processing: due to the application of alkaline lipase to remove the fillets of large |
| production method | preparation from animal tissue: the first gastric edible tissue of a calf, goat, or lamb, or pancreatic tissue of an animal. These two edible tissues were purified and extracted with water. By Aspergillus niger var. Aspergillus oryzae var. Or pseudocycle yeast (eremothium nshbyii) or the like is cultured, the fermentation broth is filtered, salted with 50% saturated ammonium sulfate solution, precipitated with acetone, and then dialyzed to crystallize. using porcine pancreas as raw material, it was obtained by crushing, degreasing with chloroform-butanol twice, extracting with ethanol and then freeze-drying. extracted from plants or prepared from Aspergillus oryzae. |
Last Update：2024-04-09 20:52:54
Supplier List
Featured ProductsSpot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86-18821248368
Email: Int06@meryer.com
Mobile: +86-18821248368
QQ: 495145328
WhatsApp: +86-18821248368
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Lipase(pocine pancreas) Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Product Name: Lipase Request for quotation
CAS: 9001-62-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
CAS: 9001-62-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Multiple SpecificationsSpot supply
Product Name: Lipase AK Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Spot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86 0571-86722205
Email: sales@chinaskyrun.com
Mobile: +8618958170122
QQ: 2531159185
Wechat: chinaskyrun
Spot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86-400-900-4166
Email: product@acmec-e.com
Mobile: +86-18621343501
QQ: 2881950922
Wechat: 18621343501
WhatsApp: +86-18621343501
Product Name: Lipase Request for quotation
CAS: 9001-62-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 9001-62-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
Featured ProductsSpot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86-18821248368
Email: Int06@meryer.com
Mobile: +86-18821248368
QQ: 495145328
WhatsApp: +86-18821248368
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Lipase(pocine pancreas) Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Product Name: Lipase Request for quotation
CAS: 9001-62-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
CAS: 9001-62-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Multiple SpecificationsSpot supply
Product Name: Lipase AK Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Spot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86 0571-86722205
Email: sales@chinaskyrun.com
Mobile: +8618958170122
QQ: 2531159185
Wechat: chinaskyrun
Spot supply
Product Name: Lipase Visit Supplier Webpage Request for quotationCAS: 9001-62-1
Tel: +86-400-900-4166
Email: product@acmec-e.com
Mobile: +86-18621343501
QQ: 2881950922
Wechat: 18621343501
WhatsApp: +86-18621343501
Product Name: Lipase Request for quotation
CAS: 9001-62-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 9001-62-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
View History